luminal type human breast cancer cell line 79 80 hcc38  (ATCC)

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: CD24/CD44 expression in monolayer and mammosphere growth conditions. ( A ) FACS profiles of the indicated cell lines grown as monolayers and stained for CD24 and CD44. ( B ) Photomicrographs of cells maintained under mammosphere growth conditions (bar=100 μ m). ( C ) FACS profiles of cells maintained under mammosphere growth conditions. ( D ) The percentages of cells with a CD24 hi phenotype in MCF7 cells grown as monolayers or mammospheres; of MDAMB468 monolayer or suspension cells with a CD24 low /CD44 hi phenotype; of the CD44 high population in MDAMB231 cells grown as monolayer or spheres. ( E ) Colony-forming efficiency (CFE) of FACS-sorted MCF7 or MDAMB468 cells on the basis of CD24/CD44 expression, or of monolayers compared with cells growing under mammosphere culture conditions before plating. Data are mean values with 95% confidence intervals; * P <0.05, n =3.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Suspension

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: ALDH activity and correlation with CD24/CD44 or colony-forming efficiency. ( A ) FACS profiles of ALDH activity in MCF7 and MDAMB468 cells grown as monolayers. The insets show fluorescence values in the presence of the inhibitor DEAB, used to set the R3 gate. ( B ) MDAMB468 cells were FACS-sorted into populations with the highest or lowest ALDH activity and stained for CD24 and CD44. ( C ) Colony-forming efficiencies of the FACS-sorted cells. ( D ) The percentages of MDAMB468 cells showing ALDH activity after growth as monolayers or under mammosphere growth conditions.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Activity Assay, Fluorescence, Staining

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: Hoechst low populations do not correlate with CD24/CD44 expression. Top panels represent data from MCF7 cells and the lower panels are from MDAMB468 cells. The left column shows FACS profiles of Hoechst staining. Cells were sorted on the basis of low fluorescence (gate R2) or were not sorted and analysed for CD24 and CD44 expression (middle and right panels).

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Fluorescence

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: Marker expression and co-localization in tumor xenografts. Immunocytochemistry for CD44 and Sox2 in MCF7 xenografts and for CD44 and ALDH1 in MDAMB468 xenografts as indicated. The image at the right-hand side of the two upper panels shows double staining of the two markers with CD44 in black and the other marker in brown. Single-label staining is counterstained with haematoxylin; no counterstain has been applied to double-labelled sections.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Marker, Expressing, Immunocytochemistry, Double Staining, Staining

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: The effects of repeated therapy on CSC markers. MCF7 or MDAMB468 cells were treated three times with Doxorubicin (Doxo), Docetaxel (Doce) or with ionizing radiation (Rx). In the top panel; the percentages of MCF7 cells showing a CD24 hi or CD24 low phenotype are shown with or without treatment (the expression levels are defined by gating on the 5% of untreated cells with the highest or lowest expression). In addition, the percentages of cells stained with CD44 or ER are shown underneath. For MDAMB468 cells, data are shown for the percentages of cells with a CD24 low /CD44 hi phenotype (third panel) or that have ALDH activity (bottom panel). Data are mean values with 95% confidence intervals, n =3 for each. * P <0.05; ** P <0.01.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Activity Assay

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: CD24/CD44 expression in monolayer and mammosphere growth conditions. ( A ) FACS profiles of the indicated cell lines grown as monolayers and stained for CD24 and CD44. ( B ) Photomicrographs of cells maintained under mammosphere growth conditions (bar=100 μ m). ( C ) FACS profiles of cells maintained under mammosphere growth conditions. ( D ) The percentages of cells with a CD24 hi phenotype in MCF7 cells grown as monolayers or mammospheres; of MDAMB468 monolayer or suspension cells with a CD24 low /CD44 hi phenotype; of the CD44 high population in MDAMB231 cells grown as monolayer or spheres. ( E ) Colony-forming efficiency (CFE) of FACS-sorted MCF7 or MDAMB468 cells on the basis of CD24/CD44 expression, or of monolayers compared with cells growing under mammosphere culture conditions before plating. Data are mean values with 95% confidence intervals; * P <0.05, n =3.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Suspension

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: ALDH activity and correlation with CD24/CD44 or colony-forming efficiency. ( A ) FACS profiles of ALDH activity in MCF7 and MDAMB468 cells grown as monolayers. The insets show fluorescence values in the presence of the inhibitor DEAB, used to set the R3 gate. ( B ) MDAMB468 cells were FACS-sorted into populations with the highest or lowest ALDH activity and stained for CD24 and CD44. ( C ) Colony-forming efficiencies of the FACS-sorted cells. ( D ) The percentages of MDAMB468 cells showing ALDH activity after growth as monolayers or under mammosphere growth conditions.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Activity Assay, Fluorescence, Staining

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: Hoechst low populations do not correlate with CD24/CD44 expression. Top panels represent data from MCF7 cells and the lower panels are from MDAMB468 cells. The left column shows FACS profiles of Hoechst staining. Cells were sorted on the basis of low fluorescence (gate R2) or were not sorted and analysed for CD24 and CD44 expression (middle and right panels).

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Fluorescence

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: Marker expression and co-localization in tumor xenografts. Immunocytochemistry for CD44 and Sox2 in MCF7 xenografts and for CD44 and ALDH1 in MDAMB468 xenografts as indicated. The image at the right-hand side of the two upper panels shows double staining of the two markers with CD44 in black and the other marker in brown. Single-label staining is counterstained with haematoxylin; no counterstain has been applied to double-labelled sections.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Marker, Expressing, Immunocytochemistry, Double Staining, Staining

Journal: British Journal of Cancer

Article Title: Lack of correlation of stem cell markers in breast cancer stem cells

doi: 10.1038/bjc.2014.105

Figure Lengend Snippet: The effects of repeated therapy on CSC markers. MCF7 or MDAMB468 cells were treated three times with Doxorubicin (Doxo), Docetaxel (Doce) or with ionizing radiation (Rx). In the top panel; the percentages of MCF7 cells showing a CD24 hi or CD24 low phenotype are shown with or without treatment (the expression levels are defined by gating on the 5% of untreated cells with the highest or lowest expression). In addition, the percentages of cells stained with CD44 or ER are shown underneath. For MDAMB468 cells, data are shown for the percentages of cells with a CD24 low /CD44 hi phenotype (third panel) or that have ALDH activity (bottom panel). Data are mean values with 95% confidence intervals, n =3 for each. * P <0.05; ** P <0.01.

Article Snippet: The luminal-type human breast cancer cell lines MCF7, T47D and ZR75-1 (steroid receptor positive); SKBR3 (HER2+); and triple negative cell lines MDAMB231 (mesenchymal) and MDAMB468 (epithelial, EGFR+) were obtained from ATCC, ECACC or DSMZ and grown in DMEM, RPMI (for T47D) or McCoys 5A (for SKBR3) with 10% fetal bovine serum (all from Invitrogen, Life Technologies Ltd., Paisley, UK) at 37 °C with 5% CO 2 .

Techniques: Expressing, Staining, Activity Assay